Anti-ENT2/SLC29A2 Rabbit Monoclonal Antibody for IHC-P, IF/ICC, ELISA - Q14542

Core Product Specifications and Parameters

Biological Background: ENT2/SLC29A2 Function and Localization

• ENT2/SLC29A2 is a member of the SLC29 family of equilibrative nucleoside transporters, encoded by the SLC29A2 gene (alias DER12, ENT2, HNP36). It is also known as HNP36 or DER12.
• Functions as a Na+-independent, passive bidirectional uniporter that mediates facilitative diffusion of various nucleosides (inosine, adenosine, uridine, thymidine, cytidine, guanosine) and nucleobases (hypoxanthine, adenine, guanine, thymine, uracil). Related references: PMID:10722669, PMID:12527552, PMID:12590919, PMID:9478986, PMID:16214850, PMID:21795683
• Additionally transports nicotinamide (PubMed:39885119) and urate (PubMed:40367900), extending its role in cellular homeostasis. Related references: PMID:39885119, PMID:40367900
• An alternative splicing isoform produces a non-functional protein that lacks cell membrane expression and cannot transport adenosine or thymidine.
• Subcellular localization is primarily at the cell membrane, with reported apical and basolateral distribution depending on cell type.
• Tissue expression includes high levels in skeletal muscle, and moderate expression in liver, lung, placenta, brain, heart, kidney, ovary, and testis. In kidney, it mediates basolateral nucleoside transport; in testis, it facilitates apical nucleoside uptake into Sertoli cells across the blood-testis barrier; and in skeletal muscle microvascular endothelium, it controls hypoxanthine flux for ROS production. Related references: PMID:12527552, PMID:23639800
• The protein is predicted to be glycosylated and phosphorylated, with a molecular weight of approximately 50 kDa (50113 Da), consistent with the reported 50kDa band.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100) of human ENT2/SLC29A2; cross-reactivity with mouse and rat suggests epitope conservation across species.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and permeabilization to detect the transmembrane protein; use positive (e.g., skeletal muscle) and negative controls.
• In ELISA, validate the antibody as capture or detection reagent; pairing with a different clone may be necessary for sandwich assays.
• Western blotting may show bands near 50 kDa, but glycosylation and phosphorylation can cause shifts; consider deglycosylation or phosphatase treatment if needed.
• Expression levels vary across tissues; high ENT2 expression in skeletal muscle makes it a recommended positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for sourcing IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target sourcing. For researchers studying nucleoside transport or developing assays targeting ENT2/SLC29A2, we provide this anti-ENT2 rabbit monoclonal antibody along with comprehensive technical and supply chain support. Contact us to explore how CamelBio can accelerate your diagnostic development from concept to clinic.