Anti-ENSA/ARPP-19e Polyclonal Antibody for WB and ELISA - O43768

Core Product Specifications and Parameters

Biological Background: ENSA Function and Localization

• Alpha-endosulfine (ENSA), also known as ARPP-19e, is a protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis.
• When phosphorylated at Ser-67 during mitosis, ENSA interacts with the regulatory subunit PPP2R2D (PR55-delta), leading to PP2A inactivation, which is essential to maintain high cyclin-B1-CDK1 activity during M phase (By similarity).
• ENSA also acts as a stimulator of insulin secretion by interacting with the sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea binding and reducing K(ATP) channel currents.
• Localizes to the cytoplasm.
• Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.
• Post-translational modifications include phosphorylation and acetylation; involved in cell cycle, mitosis, and protein phosphatase inhibition.
• Alternative splicing yields multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human ENSA; consider peptide blocking experiments to verify binding specificity.
• For Western blot, expect a band near 13 kDa under reducing conditions; validate with appropriate positive and negative controls.
• For ELISA, perform a titration experiment to determine the optimal working concentration for your assay format.
• As cross-reactivity with mouse is reported, the antibody may be used in mouse model studies, but always confirm reactivity in your specific mouse tissue/cell line.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined access to IVD raw materials, from validated antibody pairs to optimized recombinant proteins, supporting research on cell cycle regulation and metabolic signaling. Whether you need this anti-ENSA polyclonal antibody, bulk ancillary reagents, or sourcing of rare targets like PP2A regulators, our team bridges the gap from concept to clinic with technical consultation and reliable supply.