Anti-ENPP5 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q9UJA9

Core Product Specifications and Parameters

Biological Background: ENPP5 Function and Localization

• ENPP5 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 5), also known as NPP5, is a transmembrane glycoprotein that belongs to the ENPP enzyme family.
• ENPP5 preferentially hydrolyzes NAD, while it lacks significant activity toward nucleotide di- and triphosphates and does not exhibit lysophospholipase D activity.
• The enzyme is suggested to play a role in neuronal cell communication, although its precise physiological substrates remain to be fully characterized.
• ENPP5 localizes to the plasma membrane and can be shed into the extracellular space; its structure includes a zinc-binding catalytic domain and a single transmembrane helix.
• The protein has been identified in proteomics studies, and its 3D structure is available (entry Q9UJA9).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 378–477) of human ENPP5, which may confer epitope specificity for folded and membrane-embedded forms; for IF/ICC, this intracellular-facing region may require permeabilization.
• In WB, a band at approximately 55 kDa is expected; consider including a peptide blocking control to confirm specificity.
• For ELISA, the synthetic immunogen peptide can serve as a positive control to validate assay sensitivity.
• For IF/ICC, test both fixation and permeabilization conditions to optimize antigen accessibility, especially given the enzyme’s membrane localization.
• As with any antibody, performance should be validated in the relevant cell or tissue system under actual experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and biomedical companies with a one-stop platform for sourcing high-quality IVD raw materials, including monoclonal antibodies like this anti-ENPP5 clone. Our supply network includes validated antibody pairs, bulk ancillary reagents, and optimized expression systems, supporting projects from discovery through clinical development. For rare targets such as ENPP5 involved in neuronal signaling, CamelBio offers rapid sourcing and technical consulting to accelerate your assay development.