Anti-ENPP3 Polyclonal Antibody for WB, ELISA - O14638

Core Product Specifications and Parameters

Biological Background: ENPP3 Function and Localization

• ENPP3 (Ectonucleotide pyrophosphatase/phosphodiesterase family member 3), also known as CD203c, NPP3, PDNP3, and PD-IBETA, is a type II transmembrane glycoprotein and member of the ENPP family of extracellular nucleotide hydrolases.
• As a nucleotide pyrophosphatase/phosphodiesterase, ENPP3 hydrolyzes extracellular nucleotides, including ATP, GTP, UTP, and CTP, thereby regulating purinergic signaling and extracellular nucleotide pools. Related references: PMID:29717535, PMID:9344668
• ENPP3 can also degrade 2',3'-cGAMP, a second messenger that activates the STING pathway, thus acting as a negative regulator of type I interferon production and innate immune responses. Related references: PMID:38749434
• The enzyme limits mast cell and basophil activation during inflammation and chronic allergic responses by eliminating extracellular ATP, a key autocrine signaling molecule for these cells.
• Additionally, ENPP3 hydrolyzes dinucleoside polyphosphates, including vasoactive adenosine polyphosphates, suggesting a role in vascular tone regulation. Related references: PMID:12846830
• In vitro, ENPP3 displays alkaline phosphodiesterase activity, cleaving phosphodiester bonds under alkaline conditions. Related references: PMID:11342463
• Subcellularly, ENPP3 is localized to the plasma membrane, with enrichment at apical membranes in polarized cells, and can be shed as a secreted form detected in serum.
• Tissue-specific expression includes liver bile ducts, prostate, uterus, and basophils; serum levels have been associated with certain diseases.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning amino acids 646–875 of human ENPP3, corresponds to the C-terminal extracellular domain. This design may enable detection of both full-length transmembrane and shed soluble forms of ENPP3.
• For Western blot (WB), a band around 100 kDa is expected; consider validating the antibody in relevant human, mouse, or rat tissue lysates or cell lines known to express ENPP3 (e.g., basophils, prostate, liver).
• In ELISA applications, the antibody can be used either as a detection or capture reagent. Optimization of coating concentrations and detection conditions is recommended for best sensitivity.
• Cross-reactivity with mouse and rat ENPP3 is reported, but sequence homology in the immunogen region should be verified for these species; testing in the target species’ samples is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive sourcing portal for IVD raw materials. This anti-ENPP3 rabbit polyclonal antibody supports research into nucleotide metabolism and immune regulation, and CamelBio can additionally supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for your assay development needs.