Anti-eNOS Monoclonal Antibody for WB, IHC-P, ELISA - P29474

Core Product Specifications and Parameters

Biological Background: eNOS Function and Localization

• eNOS (NOS3) generates nitric oxide (NO), a gaseous signaling molecule that relaxes vascular smooth muscle via cGMP. Related references: PMID:1378832
• NO from eNOS also drives VEGF-induced coronary angiogenesis and facilitates platelet-mediated clotting.
• The gene NOS3 produces two isoforms; isoform 2 lacks catalytic activity and acts as a dominant-negative regulator by heterodimerizing with isoform 1.
• eNOS localizes primarily to caveolae at the plasma membrane, but also associates with the Golgi apparatus, cytoskeleton, and cytoplasm.
• It is expressed in platelets, placenta, liver, and kidney.
• eNOS is a multidomain enzyme requiring FAD, FMN, NADPH, heme, calcium, and calmodulin for activity, and is regulated by myristoylation, palmitoylation, and phosphorylation.
• Belongs to the nitric oxide synthase family; its molecular weight is approximately 133 kDa.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal segment (aa 1035–1110) of human eNOS, which may distinguish the active full-length isoform from truncated variants; confirm specificity using isoform-specific assays.
• For Western blotting, a band at ~133 kDa is anticipated; include endothelial lysates (e.g., HUVEC) as positive controls and use appropriate reducing conditions.
• In immunohistochemical (paraffin) analysis, antigen retrieval (e.g., citrate buffer, pH 6.0) and tissues with high endogenous eNOS expression (e.g., placenta, kidney) are recommended as initial validation steps.
• ELISA performance depends on the coating format and detection system; cross-reactivity with rat and mouse eNOS allows flexibility in species-matched assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap between concept and clinic by providing diagnostic manufacturers and laboratories with a comprehensive portfolio of IVD raw materials. For researchers investigating endothelial nitric oxide signaling and vascular pathophysiology, we offer validated monoclonal antibodies like this anti-eNOS clone alongside complementary polyclonal antibodies, recombinant proteins, and ancillary reagents in bulk volumes. Our team also specializes in sourcing rare-target raw materials to accelerate assay development.