Anti-ENO3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P13929

Core Product Specifications and Parameters

Biological Background: Beta-enolase (ENO3) Function and Localization

• ENO3 encodes beta-enolase (2-phospho-D-glycerate hydro-lyase), a glycolytic enzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate, functioning in both glycolysis and gluconeogenesis.
• Beta-enolase is also known as muscle-specific enolase (MSE) and skeletal muscle enolase, and is a member of the enolase family.
• In striated muscle, beta-enolase forms homodimers (beta/beta) or heterodimers with alpha-enolase (alpha/beta); in other tissues, alpha/alpha homodimers predominate, while gamma-enolase forms neuron-specific dimers.
• The protein plays a role in striated muscle development and regeneration, highlighting its importance beyond basic metabolism.
• Subcellularly, beta-enolase localizes to the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation, and the protein binds magnesium as a cofactor.
• Clinically, mutations in ENO3 are associated with glycogen storage disease type 13 (GSD13), a metabolic myopathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human ENO3, which shares high homology with mouse and rat orthologs, supporting the observed cross-reactivity.
• For Western blot (WB), consider using tissue lysates from skeletal muscle or heart (striated muscle) where ENO3 is highly expressed; the antibody may detect bands around 42-47 kDa, potentially reflecting isoforms or post-translational modifications.
• For immunofluorescence/immunocytochemistry (IF/ICC), validate the antibody in fixed and permeabilized cells or tissue sections from muscle, expecting cytoplasmic staining.
• For ELISA, utilize the antibody in capture/detection formats; note that peptide competition assays can confirm specificity.
• As a monoclonal antibody, lot-to-lot consistency is expected, but optimization of working dilutions and buffer conditions is recommended for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this anti-ENO3 rabbit monoclonal antibody to support diagnostic development and research in muscle metabolism and glycogen storage disorders. As your one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for IVD manufacturing. For rare or custom targets, our team provides dedicated raw-material sourcing to bridge your concept to clinic.