Anti-ENO2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P09104

Core Product Specifications and Parameters

Biological Background: ENO2 Function and Localization

• ENO2 encodes gamma-enolase, also known as neuron-specific enolase (NSE), a key isoenzyme in the enolase family alongside alpha (ENO1) and beta (ENO3) isoforms.
• Gamma-enolase catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis.
• In the central nervous system, ENO2 exhibits neurotrophic and neuroprotective properties by binding to neocortical neurons in a calcium-dependent manner, promoting cell survival.
• The enzyme forms homodimers or heterodimers; the gamma/gamma homodimer and alpha/gamma heterodimer are predominantly expressed in neurons, while alpha/alpha is found in most adult tissues.
• Subcellularly, ENO2 localizes primarily to the cytoplasm and can associate with the cell membrane.
• Post-translational modifications include acetylation, phosphorylation, hydroxylation, and ubiquitin-like conjugation, reflecting its regulatory complexity.
• ENO2 is widely used as a clinical marker for neuroendocrine tumors and neuronal injury due to its tissue-restricted expression.

Experimental Guidance and Technical Tips

• For Western blotting, expect a specific band around 47 kDa; optimize protein loading and antibody dilution using a positive control such as human brain lysate.
• For immunofluorescence/immunocytochemistry, include a permeabilization step to detect the primarily cytoplasmic localization of ENO2; consider co-staining for neuronal markers.
• In immunoprecipitation, validate the antibody's performance in your specific cell line or tissue extract, and include appropriate isotype controls.
• The confirmed cross-reactivity with mouse and rat enables this antibody to be used in rodent neuronal models; however, verify reactivity in your specific experimental context.
• For ELISA, use a standard sandwich format with appropriate blocking to minimize background; pairing with a detection antibody may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined sourcing pathway for high-quality IVD raw materials, including this anti-ENO2 polyclonal antibody for neuroscience and metabolism applications. Beyond single antibodies, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, helping you bridge from concept to clinic efficiently.