Anti-ENO1 Rabbit Monoclonal Antibody for WB, ELISA - P06733

Core Product Specifications and Parameters

Biological Background: Alpha-enolase Function and Localization

• Enzymatic role in glycolysis and gluconeogenesis: Alpha-enolase catalyzes the reversible conversion of 2-phosphoglycerate to phosphoenolpyruvate, a critical step in energy metabolism (PubMed:1369209, PubMed:29775581). Related references: PMID:1369209, PMID:29775581
• Multifunctional moonlighting protein: Beyond metabolism, alpha-enolase participates in growth control, hypoxia tolerance, and allergic responses (PubMed:10802057, PubMed:12666133, PubMed:2005901). It also acts as a cell-surface plasminogen receptor, promoting fibrinolysis, and stimulates immunoglobulin production (PubMed:12666133, PubMed:1369209). Related references: PMID:10802057, PMID:12666133, PMID:2005901, PMID:1369209
• Transcriptional regulation and tumor suppression: Alpha-enolase binds the c-myc promoter and functions as a transcriptional repressor, with possible tumor suppressor activity.
• Subcellular distribution: Localizes to the cytoplasm, cell membrane, nucleus, and also at the M line of sarcomeres in myofibrils.
• Tissue-specific expression patterns: The alpha/alpha homodimer is broadly expressed in embryonic and most adult tissues. Heterodimers with beta or gamma enolase are found in striated muscle and neurons, respectively.
• Post‑translational modifications and structural features: Contains acetylation, phosphorylation, and ubiquitination sites; requires magnesium as a metal cofactor; and forms isopeptide bonds. Crystal structures are available.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N‑terminal region (aa 1–100), which may facilitate recognition of the full‑length protein and certain isoforms. Verify in your sample system.
• Predicted molecular weight is ~47 kDa; use appropriate gel percentages for Western blotting around this range.
• Cross‑reactivity with mouse and rat makes this antibody suitable for multi‑species studies; however, always confirm reactivity in your specific models.
• For ELISA, consider pairing with a detection antibody against a distinct epitope if developing a sandwich assay.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supplies high‑quality IVD raw materials, including antibodies like this anti‑ENO1 clone, to support diagnostic developers and researchers studying glycolysis, cancer metabolism, and plasminogen‑related pathways. We offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents to streamline your assay development. Reach out to leverage our end‑to‑end sourcing capabilities for rare targets and custom scale‑up needs.