Anti-Engrailed 1 Monoclonal Antibody for WB - Q05925

Core Product Specifications and Parameters

Biological Background: Engrailed 1 Function and Localization

• Homeobox protein engrailed-1 (EN1) is a transcription factor that plays a critical role in embryonic development.
• EN1 is required for proper formation of the apical ectodermal ridge, a signaling center essential for limb outgrowth.
• It is involved in correct dorsal-ventral patterning of the limb, ensuring proper limb structure.
• As a homeobox protein, EN1 binds specific DNA sequences to regulate gene expression during development.
• The protein localizes to the nucleus, consistent with its function as a transcription factor.
• Associated keywords include developmental protein, DNA-binding, homeobox, and nucleus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 293-392) of human Engrailed 1; consider this when assessing antibody specificity in other species or isoforms.
• For Western blotting, a band near 40 kDa is anticipated; validate in your sample system using appropriate controls.
• The antibody cross-reacts with mouse and rat orthologs, making it suitable for comparative studies across these species.
• For ELISA applications, optimize the coating antigen and antibody dilution to achieve the desired sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. This anti-Engrailed 1 monoclonal antibody is one of many options available for developmental biology and transcription factor research. Our sourcing network can also supply validated antibody pairs, bulk ancillary reagents, and custom raw-material procurement for rare targets.