Anti-Endothelin 1 (ET1, PPET1) Rabbit Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P05305

Core Product Specifications and Parameters

Biological Background: Endothelin 1 Function and Localization

• Endothelin-1 (ET-1), encoded by the EDN1 gene, belongs to the endothelin family and is initially synthesized as preproendothelin-1 (PPET1), a 212-amino acid precursor processed into active peptides through cleavage on paired basic residues and disulfide bond formation.
• It acts as an endothelium-derived vasoconstrictor peptide and a probable ligand for G protein-coupled receptors EDNRA and EDNRB, triggering PTK2B, BCAR1, BCAR3, and GTPase RAP1 and RHOA signaling in glomerular mesangial cells.
  Related references: PMID:19086031
• Additionally, ET-1 binds the DEAR/FBXW7-AS1 receptor, expanding its functional repertoire beyond classical endothelin receptors.
  Related references: PMID:17446437
• Through ROCK signaling, ET-1 promotes mesenteric arterial wall remodeling, facilitating NFATC3 nuclear translocation and transcription of smooth muscle hypertrophy markers such as ACTA2.
• The protein is secreted and shows tissue-specific expression in lung, placental stem villi vessels, and cultured placental vascular smooth muscle cells, highlighting its roles in respiratory and reproductive vascular physiology.
• Key post-translational processing features include cleavage on pair of basic residues and the formation of disulfide bonds, as annotated; keywords also denote its vasoconstrictor and reference proteome status.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 18-212 of the human Endothelin-1 preproprotein (NP_001946.3), covering the majority of the precursor sequence. This design may recognize both the full-length preproprotein and its proteolytically processed forms; consider validating detection of specific isoforms in your sample system.
• For Western blotting (WB), a band around 24 kDa corresponding to the preproprotein may be observed, but additional bands from processed peptides (e.g., big endothelin-1 ~4-5 kDa, mature ET-1 ~2.5 kDa) could appear under reducing conditions. Optimize gel percentage and transfer conditions accordingly.
• In immunocytochemistry (IF/ICC) and immunohistochemistry (IHC-P), ensure proper fixation and permeabilization for optimal epitope accessibility, and include peptide blocking controls to confirm specificity if needed.
• For ELISA applications, test antibody pairing and dilution in your own matrix system, as performance may vary with sample type and assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined pathway to high-quality IVD raw materials, comprehensive technical support, and integrated consulting—from initial concept through clinical application. This anti-Endothelin 1 polyclonal antibody supports studies in cardiovascular biology, placental function, and vascular remodeling. Beyond single reagents, CamelBio can source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials, helping you accelerate assay development and scale-up with confidence.