Anti-Endonuclease G (Endo G) Rabbit Polyclonal Antibody for WB, ELISA - Q14249

Core Product Specifications and Parameters

Biological Background: Endonuclease G (Endo G) Function and Localization

• Endonuclease G is a mitochondrial endonuclease encoded by the ENDOG gene.
• Endonuclease G preferentially cleaves double-stranded 5-hydroxymethylcytosine (5hmC)-modified DNA, with increased cutting efficiency at modified sites. Related references: PMID:25355512
• It shows high affinity for four-stranded Holliday junctions and promotes conservative recombination when the DNA is 5hmC-modified. Related references: PMID:25355512
• Through its phosphorylation-mediated interaction with YWHAG, Endonuclease G suppresses mTOR signaling and promotes autophagy via a DNA damage response. Related references: PMID:33473107
• GSK3-beta-mediated phosphorylation of Endonuclease G enhances its interaction with YWHAG, leading to mTOR pathway suppression and autophagy initiation. Related references: PMID:33473107
• Under oxidative and nitrosative stress, Endonuclease G cleaves mitochondrial DNA, inducing compensatory mtDNA replication. Related references: PMID:29719607
• Subcellular localization: Mitochondrion.
• Keyword annotations: Disulfide bond, endonuclease, hydrolase, magnesium/manganese metal-binding, phosphoprotein. Calculated molecular weight: 32620 Da.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 49-297) of human Endonuclease G, covering the nuclease domain; consider this when validating in assays.
• For Western blotting, use appropriate positive controls such as mitochondrial extracts from human, mouse, or rat cell lines or tissues.
• Validate antibody specificity in the relevant sample system, as cross-reactivity may vary among species and applications.
• For ELISA, optimize coating and detection conditions based on the target's abundance in the sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including this anti-Endo G antibody for mitochondrial and DNA repair research. Our sourcing network delivers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your assay development from concept to clinic.