Anti-Endomucin Polyclonal Antibody for WB, ELISA - Q9ULC0

Core Product Specifications and Parameters

Biological Background: Endomucin Function and Localization

• Endomucin (also known as MUC14, endomucin-2, or gastric cancer antigen Ga34) is an endothelial sialomucin glycoprotein encoded by the EMCN gene.
• It interferes with the assembly of focal adhesion complexes and inhibits interactions between cells and the extracellular matrix.
• The protein is localized to the cell membrane and can also be secreted.
• Expressed in heart, kidney, and lung tissues.
• Undergoes alternative splicing, as indicated by UniProt keywords.
• Post-translationally modified by glycosylation and phosphorylation, contributing to its mucin-like properties.

Experimental Guidance and Technical Tips

• For Western blotting, consider testing the antibody on endothelial cell lines or tissue lysates from heart, kidney, or lung, where endomucin is known to be expressed. The predicted molecular weight is approximately 27 kDa, but glycosylation may result in a higher apparent band.
• For ELISA applications, the polyclonal nature of this antibody may allow use as either a capture or detection reagent after empirical optimization of coating and dilution conditions.
• As with any polyclonal antibody, lot-to-lot consistency should be verified, and appropriate positive and negative controls should be included in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD manufacturers, providing validated antibody pairs, bulk ancillary reagents, and custom procurement of difficult-to-obtain targets such as endomucin. This endomucin rabbit polyclonal antibody is suitable for research in vascular biology, cancer, and endothelial cell function. We support scaling production and technical integration to streamline your diagnostic development workflow.