Anti-ENDOGL1/ENGL Rabbit Monoclonal Antibody for WB and ELISA - Q9Y2C4

Core Product Specifications and Parameters

Biological Background: ENDOGL1/ENGL Function and Localization

• Identified as Nuclease EXOG, mitochondrial (also known as Endonuclease G-like 1 or ENDOGL1/ENGL), encoded by the EXOG gene. Alternative names include ENGL, ENGLA, ENGLB, ENDOGL1, and ENDOGL2.
• Functions as an endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA, and 5'-3' exonuclease activity.
• Localized to the mitochondrial inner membrane, as indicated by its transit peptide and membrane association.
• Ubiquitously expressed across human tissues, reflecting a fundamental role in mitochondrial nucleic acid metabolism.
• Contains a transit peptide for mitochondrial targeting and undergoes alternative splicing to produce multiple isoforms.
• Possesses metal-binding properties, characteristic of nucleases involved in DNA processing.
• The full-length protein has a molecular weight of approximately 41 kDa, consistent with the mature mitochondrial form.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 269-368 (C-terminal region) of human ENDOGL1/ENGL (Q9Y2C4). This region may exhibit isoform-specific reactivity; consider verifying against specific splice variants if multiple isoforms are of interest.
• For Western blot (WB), mitochondrial enrichment or mild lysis buffers preserving membrane fractions may enhance detection of this membrane-associated protein. Validate the antibody in relevant human cell lines (e.g., HeLa, HEK293T) expressing endogenous EXOG.
• In ELISA applications, optimize coating conditions using recombinant protein or peptide. Confirm specificity by comparing signals from wild-type versus EXOG-knockdown samples.
• As a monoclonal antibody, lot-to-lot consistency is expected. However, always titrate the antibody for each new experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with seamless access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies like this anti-ENDOGL1/ENGL rabbit mAb. With expertise spanning mitochondrial nuclease targets, we support projects from concept to clinic by offering bulk ancillary reagents, custom immunogen design, and rare target raw-material sourcing. Leverage our integrated supply chain to accelerate your assay development with reliable, research-grade reagents.