Anti-Endo G Rabbit Monoclonal Antibody for WB, ELISA - Q14249

Core Product Specifications and Parameters

Biological Background: Endo G Function and Localization

• Endo G is a mitochondrial endonuclease encoded by the ENDOG gene, localized primarily to the mitochondrion (SUBCELLULAR LOCATION: Mitochondrion).
• It preferentially cleaves double-stranded DNA containing 5-hydroxymethylcytosine (5hmC), exhibiting enhanced cutting efficiency and site specificity for modified DNAs (PubMed:25355512). Related references: PMID:25355512
• Displays significantly higher affinity for four-stranded Holliday junctions compared to duplex and single-stranded DNAs, suggesting a role in DNA recombination (By similarity).
• Promotes conservative homologous recombination when substrates are 5hmC-modified (PubMed:25355512). Related references: PMID:25355512
• Mediates autophagy by suppressing the mTOR pathway through phosphorylation-dependent interaction with YWHAG and its endonuclease activity-induced DNA damage response (PubMed:33473107). Related references: PMID:33473107
• GSK3-beta-mediated phosphorylation enhances Endo G binding to YWHAG, which releases TSC2 and PIK3C3 from YWHAG, leading to mTOR suppression and autophagy initiation (PubMed:33473107). Related references: PMID:33473107
• Cleaves mitochondrial DNA (mtDNA) in response to oxidative and nitrosative stress, thereby inducing compensatory mtDNA replication (PubMed:29719607). Related references: PMID:29719607
• The protein contains disulfide bonds and is post-translationally regulated by phosphorylation (Keywords: Disulfide bond, Phosphoprotein).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 198–297 of human Endo G. Researchers may consider mapping this region to known functional or structural domains to predict epitope accessibility.
• For Western blot applications, Endo G migrates at approximately 33 kDa. Include appropriate positive controls (e.g., mitochondrial fractions from human, mouse, or rat cells) and validate detection in your specific sample type.
• Due to its mitochondrial localization, efficient protein extraction may require mitochondrial isolation kits or lysis buffers optimized for organelle proteins.
• In ELISA, pairing with a suitable capture/detection antibody may be considered; confirm linearity and sensitivity using recombinant Endo G or cell lysates.
• Cross-reactivity is reported for human, mouse, and rat. Validate specificity in each species, especially if sequence homology in the immunogen region varies.

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CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-Endo G clone. Whether you need antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets in the apoptosis and mitochondrial research area, CamelBio provides a streamlined procurement experience. Our one-stop approach covers every stage from concept to clinic, helping you accelerate assay development and production.