Anti-ENAH Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N8S7

Core Product Specifications and Parameters

Biological Background: ENAH Function and Localization

• Protein enabled homolog (ENAH), also known as MENA, NDPP1, and ENA, belongs to the Ena/VASP family of actin-associated proteins.
• ENAH plays a critical role in cytoskeleton remodeling and cell polarity, including axon guidance, lamellipodial and filopodial dynamics, and cell migration.
• ENAH induces the formation of F-actin rich outgrowths in fibroblasts and synergizes with BAIAP2-alpha downstream of netrin-1 (NTN1) to promote filopodia formation (By similarity).
• ENAH localizes to the cytoplasm, associates with the cytoskeleton, and is enriched at cell projections such as lamellipodia, filopodia, focal adhesions, and synapses.
• Expressed in various glandular epithelia, smooth muscle, and vascular media, ENAH is notably overexpressed in breast cancer cell lines and primary tumors, underscoring its role in cancer biology.
• ENAH undergoes alternative splicing, yielding multiple isoforms, and is regulated by phosphorylation; it also contains SH3-binding motifs for interactions with signaling proteins.
• As an actin-binding protein, ENAH contributes to the dynamic reorganization of the actin cytoskeleton essential for cellular motility and morphology.

Experimental Guidance and Technical Tips

• The immunogen spans residues 412-591 of human ENAH, a region that may confer target specificity for polyclonal detection.
• The antibody has been applied in Western blotting (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. For WB, expect a band near 67 kDa; validate loading controls and secondary antibodies in your sample system.
• Given cross-reactivity with mouse and rat, consider this antibody for rodent model studies; verify compatibility in your experimental setup.
• For IF/ICC, optimize fixation and permeabilization to preserve cytoskeletal structures and achieve clear localization at lamellipodia and filopodia.
• In ELISA, titrate both antibody and antigen to establish optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, offering validated antibodies like this ENAH polyclonal antibody to support research into cytoskeleton dynamics and cancer. Our comprehensive portfolio includes monoclonal/polyclonal antibodies, antigens, and ancillary reagents, streamlining procurement for diagnostic manufacturers and research laboratories. Leverage our expertise to source rare targets and accelerate your assay development from concept to clinic.