Anti-EMX1 Polyclonal Antibody for WB and ELISA - Q04741

Core Product Specifications and Parameters

Biological Background: EMX1 Function and Localization

• EMX1 is a homeobox transcription factor encoded by the EMX1 gene (alternative designations: Empty spiracles homolog 1, Empty spiracles-like protein 1).
• It cooperates with EMX2 to establish the boundary between the roof and archipallium during embryonic brain development, and may act together with OTX1/2 to specify cell fates in the central nervous system.
• The protein contains a DNA-binding homeobox domain and is classified as a developmental protein.
• Subcellular localization is both nuclear and cytoplasmic, consistent with its transcription factor role and potential cytoplasmic functions.
• EMX1 is predominantly expressed in the cerebral cortex.
• Alternative splicing generates multiple transcript variants (UniProt keyword: Alternative splicing).

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against a recombinant protein covering amino acids 70–190 of human EMX1. It is expected to recognize full-length EMX1 and potentially its splice isoforms; validate in your sample system.
• For Western blot, consider using human cerebral cortex lysate or EMX1-expressing cell lines. A band near 31 kDa is predicted, but alternative splicing may yield additional bands.
• For ELISA, the antibody can be used with recombinant EMX1 protein or peptide; optimize coating and detection conditions.
• This antibody cross-reacts with mouse, enabling its use in mouse brain developmental studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting, guiding projects from early concept through to clinical application. This EMX1 polyclonal antibody is part of a broad portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neuroscience and developmental biology applications, CamelBio can also assist with the sourcing of rare-target raw materials to support your assay development and diagnostic workflows.