Anti-EMSY/C11orf30 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q7Z589

Core Product Specifications and Parameters

Biological Background: EMSY Function and Localization

• Protein Identity and Isoforms: EMSY (C11orf30), also known as BRCA2-interacting transcriptional repressor EMSY, is encoded by the EMSY gene. Alternative splicing generates multiple isoforms.
• Transcriptional Repression and Chromatin Remodeling: EMSY functions as a transcriptional repressor, likely through interaction with a multiprotein chromatin remodeling complex that modifies chromatin structure to silence gene expression. Related references: PMID:14651845
• Role in DNA Repair via BRCA2 Interaction: EMSY interacts with BRCA2, suggesting a central role in the DNA repair function of BRCA2, linking chromatin regulation to genomic stability. Related references: PMID:14651845
• Nuclear Hormone Receptor Coactivation: In addition to its repressive functions, EMSY mediates ligand-dependent transcriptional activation by nuclear hormone receptors, indicating a dual regulatory role. Related references: PMID:19131338
• Subcellular Localization: EMSY localizes predominantly to the nucleus, consistent with its functions in transcription regulation and DNA repair.
• Post-translational Modifications and Keywords: According to UniProt, EMSY is a glycoprotein and phosphoprotein, and is classified under categories such as chromatin regulator, DNA damage, DNA repair, repressor, and transcription regulation.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant immunogen corresponding to amino acids 1153-1322 of human EMSY. Researchers can confirm reactivity with their target by using the relevant control lysates or cells.
• For Western blot (WB), a band at approximately 141 kDa is expected. Consider using a positive control such as HeLa or other human cell lines known to express EMSY.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimal fixation and permeabilization conditions should be determined empirically. Nuclear staining is anticipated.
• For ELISA, the antibody can be used as a capture or detection reagent. Validate pairing with other antibodies if developing a sandwich ELISA.
• Cross-reactivity is reported for human and mouse. If using in other species, verify sequence homology of the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. For projects involving EMSY and related DNA repair or chromatin biology targets, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials. Our streamlined supply chain and technical support help accelerate your assay development and commercialization.