Anti-EMR2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UHX3

Core Product Specifications and Parameters

Biological Background: EMR2 Function and Localization

• EMR2, also known as ADGRE2 or CD312, belongs to the adhesion G protein-coupled receptor (aGPCR) family and contains characteristic EGF-like repeats in its extracellular domain.
• Functions as a cell surface receptor for glycosaminoglycans, specifically binding chondroitin sulfate moieties to mediate cell attachment.
• Promotes innate immune responses by inducing granulocyte chemotaxis, degranulation, and adhesion.
• In macrophages, EMR2 activation triggers the release of pro-inflammatory cytokines IL-8 and TNF.
• Serves as a regulator of mast cell degranulation. Related references: PMID:26841242
• Localizes to the plasma membrane and is enriched in membrane ruffles, consistent with its role in cell motility and adhesion.
• Exhibits myeloid-restricted expression, with high levels in peripheral blood leukocytes, mast cells, and myeloid cell lines, and is absent from non-immune tissues.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–500 of human EMR2, covering part of the extracellular EGF-like domain; it may recognize both full-length and autocatalytically cleaved receptor forms.
• For Western blotting, expect a band around 90 kDa; note that extensive glycosylation can cause the observed molecular weight to be higher.
• For ELISA, the antibody may be used as capture or detection reagent; titration and optimization with recombinant EMR2 protein are recommended.
• Reported cross-reactivity with mouse EMR2 supports translational studies in murine models; validate with appropriate tissue or cell line controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides integrated sourcing solutions for IVD raw materials, including validated monoclonal and polyclonal antibodies, purified antigens, and ancillary reagents. For adhesion GPCR research and diagnostic development targeting EMR2, we offer bulk supply and custom sourcing to support projects from early discovery through clinical validation. Our streamlined supply chain ensures consistent lot-to-lot performance, accelerating your path from concept to clinic.