Anti-EMR1 Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q14246

Core Product Specifications and Parameters

Biological Background: EMR1 (ADGRE1) Function and Localization

• EMR1, also known as ADGRE1, TM7LN3, and EGF-like module receptor 1, encodes adhesion G protein-coupled receptor E1, a member of the adhesion GPCR family characterized by extracellular EGF-like domains.
• The receptor functions as an orphan adhesion GPCR involved in cell-cell interactions and cell adhesion, particularly within the immune system.
• EMR1 may contribute to regulatory T-cell (Treg) development, suggesting a role in immune homeostasis.
• Expression of EMR1 is highly restricted to eosinophils, indicating specialized functions in these granulocytes.
• The protein localizes to the cell membrane and contains features typical of GPCRs, including seven-transmembrane domains, calcium-binding motifs, disulfide bonds, and glycosylation sites.
• The calculated molecular weight of the full-length precursor is approximately 97.7 kDa, consistent with the observed 98 kDa band.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fusion protein spanning amino acids 477–596 (NP_001965.3), an extracellular region that may include EGF-like domains; this design supports recognition of the native receptor.
• For Western blot (WB), a band near 98 kDa is expected; consider using protease inhibitors and reduced denaturing conditions to detect the glycosylated transmembrane protein.
• In immunofluorescence (IF/ICC) and IF-P, permeabilization and antigen retrieval steps may be required to access the membrane-localized target.
• The antibody’s cross-reactivity with mouse and rat enables use in common model organisms; validate specificity using eosinophil-rich tissues or cell lines.
• For ELISA applications, pair with a matched detection antibody or use as a capture reagent after confirming binding in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting. Our EMR1/ADGRE1 polyclonal antibody supports investigations in immunology and cell adhesion, aligning with our sourcing capabilities—including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material procurement. As your one-stop partner, we streamline supply from concept to clinic.