Anti-EML4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HC35

Core Product Specifications and Parameters

Biological Background: EML4 Function and Localization

• EML4 is a microtubule-associated protein belonging to the echinoderm microtubule-associated protein-like family, also known as Restrictedly overexpressed proliferation-associated protein (Ropp 120).
• It is essential for the formation and stability of microtubules (MTs). Related references: PMID:16890222, PMID:31409757
• Required for the organization of the mitotic spindle, proper kinetochore-MT attachment, and recruitment of NUDC for mitotic progression. Related references: PMID:25789526
• Subcellularly localized to the cytoplasm, cytoskeleton, mitotic spindle, microtubule organizing center, and midbody.
• Contains coiled-coil and WD repeat domains and undergoes post-translational modifications including acetylation and phosphorylation.
• Subject to alternative splicing and chromosomal rearrangements, which can generate oncogenic fusion proteins.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 360-560 of human EML4; polyclonal nature may produce reactivity against multiple epitopes.
• Predicted molecular weight for EML4 is approximately 109 kDa by Western blot; consider using a suitable gel percentage for high-molecular-weight protein separation.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions for your sample type.
• Cross-reactivity with mouse and rat EML4 is indicated; validation in the relevant species model is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing hub for IVD raw materials, supporting diagnostic manufacturers and research laboratories with reliable antibodies, antigen pairs, and ancillary reagents. This anti-EML4 polyclonal antibody is well suited for microtubule, mitosis, and cell cycle research programs. Our supply chain expertise covers validated polyclonal and monoclonal antibodies, bulk reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.