Anti-EML3 Polyclonal Antibody for WB, ELISA - Q32P44

Core Product Specifications and Parameters

Biological Background: EML3 Function and Localization

• EML3 (Echinoderm microtubule-associated protein-like 3) is known by aliases ELP95, EMAP3, and EMAP95.
• Regulates mitotic spindle assembly, microtubule-kinetochore attachment, and chromosome separation by recruiting the HAUS augmin-like complex and TUBG1 to existing microtubules, promoting microtubule-based microtubule nucleation. Related references: PMID:30723163
• Required for proper alignment of chromosomes during metaphase. Related references: PMID:18445686
• Localizes to the cytoplasm, cytoskeleton, spindle, nucleus, and midbody, consistent with its mitotic roles.
• Contains WD repeats and a coiled-coil domain; classified under the WD repeat EML family.
• Subject to acetylation and phosphorylation, indicating regulation of its activity during the cell cycle.
• Alternatively spliced isoforms may exist.
• Identified in reference proteome studies, confirming its presence in human cellular protein datasets.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–530; consider this region for epitope analysis or blocking experiments.
• For Western blot, expect a band near 95 kDa in human samples; validate antibody specificity using appropriate positive/negative controls.
• When performing ELISA, optimize coating antigen concentration and antibody dilution for best signal-to-noise ratio.
• Not yet tested in other applications; cross-reactivity with other species is not characterized—pilot studies are advised if extending beyond human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities with integrated access to IVD raw materials, including cell-cycle and mitosis-related antibodies like this anti-EML3 polyclonal antibody. Our supply capabilities encompass validated antibody pairs, bulk ancillary reagents, and assistance in sourcing rare targets, helping you streamline assay development from concept to clinical application.