Anti-Emerin/EMD Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P50402

Core Product Specifications and Parameters

Biological Background: Emerin/EMD Function and Localization

• Emerin is encoded by the EMD gene and is also known as STA, EDMD, and LEMD5. It is a nuclear envelope protein that stabilizes and promotes the formation of a nuclear actin cortical network. Related references: PMID:15328537
• It inhibits β-catenin transcriptional activity by promoting its nuclear export through a CRM1-dependent pathway. Related references: PMID:16858403
• Emerin links centrosomes to the nuclear envelope via microtubule association, contributing to nuclear positioning. Related references: PMID:17785515
• It is required for the correct localization of non-farnesylated prelamin-A/C, a key intermediate in lamin maturation. Related references: PMID:19323649
• Together with NEMP1, emerin contributes to nuclear envelope stiffness, particularly in germ cells. Related references: PMID:32923640
• Emerin acts as a cofactor for HIV-1 infection by associating with viral DNA in the presence of BAF and viral integrase, facilitating chromatin association.
• Subcellularly, emerin localizes to the inner and outer nuclear membranes.
• Tissue expression is highest in skeletal muscle, heart, colon, testis, ovary, and pancreas, reflecting its roles in muscle and germ cell function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 150-254 of human emerin, a region likely exposed on the nucleoplasmic surface, suitable for detection in multiple assays.
• For western blot (WB), a band at ~29 kDa is expected; consider using appropriate controls and validate in your sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), ensure proper permeabilization to access the nuclear envelope; fixation conditions may affect epitope availability.
• The antibody cross-reacts with mouse and rat, enabling multi-species studies; verify reactivity in your model of interest.
• For ELISA, the antibody can be used as a capture or detection reagent; optimization of coating conditions and detection format is recommended.
• Given its role in actin and microtubule dynamics, consider co-staining with cytoskeletal markers to examine structural associations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to premium IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies targeting nuclear envelope proteins like emerin. Our portfolio spans bulk ancillary reagents and rare target sourcing, ensuring supply continuity for emerin-related chromatin, laminopathy, and muscular dystrophy research. Whether you require ready-to-use reagents or custom development, CamelBio bridges the gap from concept to clinic with reliable B2B support.