Anti-EME1 Polyclonal Antibody for WB and ELISA - Q96AY2

Core Product Specifications and Parameters

Biological Background: EME1 Function and Localization

• EME1, also known as MMS4L or SLX2A, is the non-catalytic subunit of the MUS81-EME1 structure-specific endonuclease complex, essential for maintaining genomic stability by processing DNA intermediates such as 3'-flaps and nicked Holliday junctions. Related references: PMID:12686547, PMID:12721304, PMID:14617801, PMID:24733841, PMID:35290797
• The MUS81-EME1 heterodimer exhibits limited endonuclease activity on 5' flaps and nicked double-stranded DNA, contributing to DNA repair and recombination processes. Related references: PMID:24733841, PMID:35290797
• During prometaphase, MUS81-EME1 resolves mitotic recombination intermediates, including Holliday junctions, to ensure accurate chromosome segregation. Related references: PMID:14617801, PMID:24813886
• EME1 localizes to the nucleus and nucleolus, consistent with its role in DNA metabolism.
• Post-translational modifications of EME1 include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, potentially regulating its activity.
• Alternative splicing produces multiple EME1 isoforms, adding functional diversity.

Experimental Guidance and Technical Tips

• For Western blot detection, the predicted molecular weight of human EME1 is 63 kDa; validate band specificity in relevant cell or tissue lysates.
• Given the polyclonal nature of this antibody, it may recognize multiple EME1 isoforms; consider confirming isoform identity via additional methods if needed.
• The antibody is validated for ELISA and WB; for other applications, perform pilot experiments to determine suitability.
• Due to reported cross-reactivity with mouse and rat, it can be used for comparative studies; however, verify cross-reactivity in your specific model system.
• Optimize blocking and antibody dilution conditions using the relevant sample matrix to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw-material procurement by offering validated antibody pairs, bulk ancillary reagents, and access to rare targets like structure-specific endonucleases for DNA repair research. As a comprehensive sourcing partner, we support diagnostic manufacturers from concept to clinic with optimized polyclonal antibodies, technical consultation, and reliable supply chains.