Anti-EMC8 Polyclonal Antibody for WB, ELISA - O43402

Core Product Specifications and Parameters

Biological Background: EMC8 Function and Localization

• EMC8 is a subunit of the endoplasmic reticulum membrane protein complex (EMC) that enables energy-independent insertion of nascent membrane proteins, preferentially accommodating those with weakly hydrophobic or destabilizing transmembrane domains (PubMed:29242231, PubMed:29809151, PubMed:30415835). Related references: PMID:29242231, PMID:29809151, PMID:30415835
• The EMC is involved in the cotranslational insertion of multi-pass membrane proteins where stop-transfer sequences become ER membrane-spanning helices, and is also required for post-translational insertion of tail-anchored proteins (PubMed:29809151, PubMed:30415835, PubMed:29242231). Related references: PMID:29809151, PMID:30415835, PMID:29242231
• By mediating proper cotranslational insertion with N-exo topology, EMC8 controls the topology of multi-pass proteins such as G protein-coupled receptors (PubMed:30415835). Related references: PMID:30415835
• EMC8 is localized to the endoplasmic reticulum membrane.
• The protein is expressed in various tissues (pancreas, liver, heart, lung, kidney, brain, skeletal muscle, placenta) with highest levels in pancreas and moderate in heart, skeletal muscle, and placenta.
• Keywords associated with EMC8 include alternative splicing, 3D-structure, and proteomics identification, reflecting its structural characterization and potential isoform diversity.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the full-length human EMC8 (aa 1-210). For Western blotting, consider that the predicted molecular weight is approximately 24 kDa, though post-translational modifications or alternative splicing may yield multiple bands. Validate band specificity with relevant controls.
• For ELISA applications, this polyclonal antibody can be utilized as capture or detection reagent. Given the reported cross-reactivity with human and mouse, it is advisable to validate performance in the specific sample matrix and species of interest.
• As a polyclonal antibody, lot-to-lot variability can occur; it is recommended to include internal reference samples in each assay for consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive one-stop supply chain for IVD raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. Our portfolio supports studies on endoplasmic reticulum membrane protein complexes and membrane protein biogenesis, with reliable monoclonal and polyclonal antibodies tailored for assay development. For specialized or rare targets like EMC8, CamelBio leverages its sourcing network to deliver consistent, high-quality materials to accelerate your research from concept to clinic.