Anti-EMC4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q5J8M3

Core Product Specifications and Parameters

Biological Background: EMC4 Function and Localization

• EMC4, also known as PIG17 or TMEM85, is a subunit of the endoplasmic reticulum membrane protein complex (EMC), a multi-protein insertase that facilitates the biogenesis of membrane proteins.
• The EMC mediates energy-independent insertion of newly synthesized membrane proteins into the ER membrane, with a preference for substrates containing weakly hydrophobic or destabilizing transmembrane domains. Related references: PMID:29242231, PMID:29809151, PMID:30415835
• It is essential for the cotranslational insertion of multi-pass membrane proteins, ensuring proper stop-transfer membrane-anchor sequence integration into ER membranes. Related references: PMID:29809151, PMID:30415835
• Additionally, EMC4 is required for the post-translational insertion of tail-anchored (TA) proteins into the ER membrane. Related references: PMID:29242231, PMID:29809151
• By mediating proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, the EMC controls the topological orientation of multi-pass membrane proteins, such as G protein-coupled receptors (GPCRs). Related references: PMID:30415835
• EMC4 localizes to the endoplasmic reticulum membrane. Alternative splicing generates isoforms with distinct tissue expression—isoform 1 is expressed in brain and heart, while isoform 2 is expressed in heart.
• The protein is subject to acetylation and phosphorylation, and has been identified in proteomics analyses; it is annotated with keywords including alternative splicing, apoptosis, and transmembrane domains.

Experimental Guidance and Technical Tips

• Immunogen design: The recombinant immunogen covers amino acids 1–100 of human EMC4 (N-terminal region). Consider verifying antibody reactivity against the full-length endogenous protein, as this region may include both structured and disordered elements.
• Western blot: The antibody detects bands around 15 kDa, 16 kDa, and 20 kDa, likely reflecting post-translational modifications or isoform differences. Validate these patterns in your specific sample system.
• IHC-P: Antigen retrieval and detection conditions should be optimized for each tissue type. No single protocol is universally applicable; pilot studies are recommended.
• ELISA: Suitable for standard assay formats; however, coating conditions and detection ranges must be empirically determined.
• Cross-reactivity: The antibody cross-reacts with mouse and rat EMC4, but optimal working dilutions may vary between species. Titration is advised.
• General note: All experiments should include appropriate positive and negative controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated anti-EMC4 rabbit monoclonal antibodies. As a comprehensive sourcing partner, we offer customized supply solutions spanning antibody development, bulk ancillary reagent provision, and rare target raw material procurement to accelerate your assay development from concept to clinical application.