Anti-EMC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q8N766

Core Product Specifications and Parameters

Biological Background: EMC1 Function and Localization

• EMC1 is a core subunit of the endoplasmic reticulum membrane protein complex (EMC).
• The EMC facilitates the energy-independent insertion of membrane proteins, preferentially those with weak hydrophobic TMDs or destabilizing features. Related references: PMID:29242231, PMID:29809151, PMID:30415835
• It is required for both co-translational insertion of multi-pass membrane proteins and post-translational insertion of tail-anchored proteins. Related references: PMID:29242231, PMID:29809151, PMID:30415835
• EMC1 controls the topology of multi-pass proteins such as GPCRs by ensuring N-exo orientation of N-terminal transmembrane domains. Related references: PMID:30415835
• The protein localizes to the ER membrane, with a signal sequence and a large luminal domain; it is glycosylated and contains disulfide bonds.
• Alternative splicing generates isoforms; mutations in EMC1 are associated with neurodegenerative disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 885-965 near the C-terminus; this region may be used for epitope mapping during assay design.
• This polyclonal antibody is suitable for WB, IHC-P, and ELISA in human samples; specificity should be verified in the relevant experimental system.
• For WB, the predicted molecular weight is 112 kDa; glycosylation may cause band shifts—validate with appropriate controls.
• In IHC-P, optimize antigen retrieval conditions to account for the protein's membrane localization.
• When using ELISA, consider testing against recombinant protein or peptide to confirm immunoreactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with streamlined access to essential IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. For research on ER membrane protein complex and its role in protein biogenesis, CamelBio offers this anti-EMC1 antibody alongside technical consultation and custom sourcing solutions to accelerate your assay development from concept to clinical application.