Anti-EMB (Embigin/GP70) Rabbit Polyclonal Antibody for WB and ELISA - Q6PCB8

Core Product Specifications and Parameters

Biological Background: Embigin Function and Localization

• Embigin is a transmembrane glycoprotein that plays a critical role in the outgrowth of motoneurons and the formation of neuromuscular junctions.
• Following muscle denervation, EMB promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology.
• EMB delays the retraction of terminal sprouts after re-innervation of denervated endplates, contributing to synaptic recovery.
• This protein may also be involved in targeting monocarboxylate transporters (SLC16A1, SLC16A6, SLC16A7) to the cell membrane.
• Subcellularly, Embigin localizes to the cell membrane and synapse, consistent with its role at neuromuscular junctions.
• Key structural features include immunoglobulin domains, disulfide bonds, and potential alternative splicing and phosphorylation sites.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and include appropriate positive control lysates (e.g., human skeletal muscle or neuroblastoma cells) to validate band specificity.
• Since the antibody is a polyclonal, minor background bands may be observed; optimize blocking and antibody dilution for your sample type.
• For ELISA, optimize coating antigen concentration and detection conditions based on standard indirect ELISA protocols.
• Cross-reactivity is reported for human; performance in other species has not been tested and should be validated independently.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with efficient access to high-quality IVD raw materials, including this anti-EMB polyclonal antibody targeting Embigin—a key player in neuromuscular biology. Whether you need validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, or assistance sourcing rare-target raw materials, our integrated supply chain and technical support help streamline your development from concept to clinic.