Anti-ELSPBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q96BH3

Core Product Specifications and Parameters

Biological Background: ELSPBP1 Function and Localization

• ELSPBP1 (Epididymal sperm-binding protein 1), also known as Epididymal secretory protein 12 (hE12), is encoded by the ELSPBP1 (E12) gene.
• Binds to spermatozoa upon ejaculation and may play a role in sperm capacitation (By similarity).
• Exhibits phosphorylcholine-binding activity.
• Belongs to the epididymal sperm-binding protein family.
• Localized as a secreted protein; detected in cauda epididymidal fluid and on the sperm membrane.
• Contains disulfide bond(s) and is a glycoprotein, as indicated by UniProt keywords.
• Involved in fertilization processes (UniProt keyword: Fertilization).
• This protein is part of the reference proteome and has been identified by proteomic analysis.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length ELSPBP1 protein (aa 1-223), supporting detection of both native and denatured forms. Consider this when selecting assay conditions.
• This antibody is suitable for Western blot (WB) and ELISA. For WB, validate in relevant biological samples such as human cauda epididymidal fluid or sperm lysates.
• To ensure specificity, use appropriate negative controls (e.g., tissues with low or absent ELSPBP1 expression) and consider pre-adsorption tests if necessary.
• As ELSPBP1 is a secreted glycoprotein, sample preparation for WB may benefit from deglycosylation if band shifts or multiple bands are observed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, supporting diagnostic manufacturers and research institutes from concept to clinic. This anti-ELSPBP1 polyclonal antibody is ideal for reproductive biology and fertility research, including sperm capacitation studies. Beyond this product, CamelBio can supply validated antibody pairs, bulk ancillary reagents, and assist with sourcing rare-target raw materials—all tailored to streamline your assay development pipeline.