Anti-ELSPBP1 Polyclonal Antibody for WB, IF-P, ELISA - Q96BH3

Core Product Specifications and Parameters

Biological Background: ELSPBP1 Function and Localization

• ELSPBP1 (Epididymal sperm-binding protein 1), also known as Epididymal secretory protein 12 (hE12), is a secreted glycoprotein encoded by the ELSPBP1 gene (GeneID: 64100).
• It is expressed in the cauda epididymis and detected in epididymal fluid and on the sperm membrane post-ejaculation.
• Functionally, ELSPBP1 binds to spermatozoa and is implicated in sperm capacitation, a critical maturation step for fertilization.
• It exhibits phosphorylcholine-binding activity, which may mediate interactions with sperm membrane phospholipids.
• Classified under fertilization-related proteins, it contains a signal peptide, disulfide bonds, and repeat regions, indicating a structured secretory protein.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fragment covering amino acids 110-223 of human ELSPBP1; performance should be validated in the relevant species and sample type (e.g., epididymal fluid, sperm lysates).
• Cross-reactivity with mouse and rat is indicated, but users should verify specificity in their assay system, especially for applications like IF-P.
• For WB, consider using positive control lysates from cauda epididymis or sperm samples; the expected band size is approximately 26 kDa (precursor) or possibly processed forms.
• ELISA and immunofluorescence protocols may require optimization of blocking and detection conditions due to the secreted nature of the target.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated polyclonal antibodies like anti-ELSPBP1 for fertility research. We offer bulk ancillary reagents, optimized antibody pairs, and rare target sourcing to support your assay development from concept to clinic. Count on us as your end-to-end partner in reproductive biology research supplies.