Anti-ELP5 Rabbit Polyclonal Antibody for WB and ELISA - Q8TE02

Core Product Specifications and Parameters

Biological Background: ELP5 Function and Localization

• ELP5 (Elongator complex protein 5), also known as Dermal papilla-derived protein 6 (DERP6) or S-phase 2 protein, is a core component of the elongator complex essential for tRNA modifications.
• The elongator complex catalyzes the formation of carboxymethyluridine at wobble position 34 in tRNAs, generating mcm5U, mcm5s2U, and ncm5U modifications critical for translational fidelity.
  Related references: PMID:29332244
• ELP5 participates in cell migration, as indicated by similarity data.
• Subcellular localization encompasses both the nucleus and the cytoplasm, aligning with its role in tRNA processing.
• Tissue expression is ubiquitous, with notably high levels in heart, brain, liver, skeletal muscle, and testis.
• Post‑translational modifications include phosphorylation; the protein is also subject to alternative splicing and alternative initiation (UniProt keywords).
• Keywords: tRNA processing, Nucleus, Cytoplasm, Phosphoprotein, Alternative splicing, Alternative initiation, Reference proteome.

Experimental Guidance and Technical Tips

• For Western blotting, an anticipated band near 35 kDa may be observed; however, validation in the relevant sample system is recommended.
• Given its polyclonal nature, this antibody is suitable for ELISA applications; consider optimizing coating and detection conditions for best results.
• The recombinant immunogen (aa 17–316) covers most of the full‑length sequence, suggesting broad epitope recognition.
• Confirm cross‑reactivity with mouse samples before large‑scale experiments, as reactivity may vary depending on lysate preparation and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with integrated access to IVD raw materials, from validated antibody pairs and optimized polyclonal/monoclonal antibodies to bulk ancillary reagents. For projects exploring tRNA modification or cell migration, we support customized sourcing of rare targets and technical consultation. Let CamelBio efficiently bridge your concept to clinical application.