Anti-ELOVL5 Polyclonal Antibody for WB and ELISA - Q9NYP7

Core Product Specifications and Parameters

Biological Background: Very Long Chain Fatty Acid Elongase 5 Function and Localization

• ELOVL5 encodes the very long chain fatty acid elongase 5, a key enzyme in the elongation cycle of long-chain fatty acids, and multiple isoforms are produced through alternative splicing.
• It catalyzes the first and rate-limiting reaction of the long-chain fatty acids elongation cycle in the endoplasmic reticulum, adding two carbons per cycle to long- and very long-chain fatty acids (VLCFAs).
  Related references: PMID:10970790 PMID:20937905
• The enzyme acts as a condensing enzyme with high specificity toward polyunsaturated acyl-CoA, particularly C18:3(n-6) acyl-CoA, and is involved in producing monounsaturated and polyunsaturated VLCFAs that serve as precursors of membrane lipids and lipid mediators.
  Related references: PMID:10970790 PMID:20937905
• ELOVL5 is localized primarily to the endoplasmic reticulum membrane and is also found in cell projections such as dendrites.
• Ubiquitously expressed, it shows high expression in the adrenal gland and testis, with weaker levels in prostate, lung, and brain, and is detected in the cerebellum.
• In the absence of essential linoleic and alpha-linolenic acids, ELOVL5 participates in the synthesis of Mead acid from oleic acid, an alternative pathway for polyunsaturated fatty acid production.
• Mutations in ELOVL5 are associated with spinocerebellar ataxia type 38 (SCA38), a neurodegenerative disorder, highlighting its critical role in neural lipid metabolism.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity and function.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from tissues with high ELOVL5 expression such as adrenal gland or testis. The predicted molecular weight is approximately 35kDa; optimize gel percentage and transfer conditions accordingly.
• For ELISA, the polyclonal nature may provide broad reactivity; validate the antibody using recombinant ELOVL5 protein or native samples and include appropriate negative controls.
• The immunogen corresponds to amino acids 247–326 in the C-terminal region; verify reactivity with specific isoforms if alternative splicing is a concern in your experimental system.
• As a rabbit polyclonal antibody, confirm consistency across different batches for reproducibility in long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive supply chain for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. The ELOVL5 polyclonal antibody supports research in lipid metabolism and spinocerebellar ataxia. Leverage our technical consulting to accelerate your assay development from concept to clinic.