Anti-ELOVL4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9GZR5

Core Product Specifications and Parameters

Biological Background: ELOVL4 Function and Localization

• ELOVL4 (ELOVL fatty acid elongase 4) is a very long chain fatty acid elongase, also known as 3-keto acyl-CoA synthase ELOVL4, and is implicated in Stargardt disease type 3 (STGD3) and other disorders.
• It catalyzes the first and rate-limiting step of the cycle that adds two carbon units to long-chain and very long-chain fatty acids, a process essential for the synthesis of saturated and polyunsaturated VLCFAs.
• ELOVL4 is an integral endoplasmic reticulum membrane protein, consistent with its role in lipid elongation occurring at the ER membrane.
• The enzyme produces VLC-SFA and PUFA that serve as precursors for membrane lipids and signaling mediators, highlighting its importance in cellular membrane composition and lipid mediator biosynthesis.
• It may play a critical role in early brain and skin development, based on functional studies and disease associations (e.g., ichthyosis and intellectual disability).
• ELOVL4 is highly expressed in the retina, with lower expression in the brain, thymus, testis, small intestine, ovary, and prostate; minimal expression in heart, lung, liver, or leukocytes.
• Known disease variants are linked to Stargardt disease, spinocerebellar ataxia, ichthyosis, and intellectual disability, reflecting its crucial neurodevelopmental and dermatological functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminus (amino acids 1-100) of human ELOVL4; for critical applications, consider peptide blocking to confirm signal specificity.
• For Western blot, expect a band near 37 kDa; validate in appropriate tissue or cell lysates, such as retinal or brain extracts, which are known to express ELOVL4.
• In IF/ICC, anticipated localization is the endoplasmic reticulum; use co-staining with ER markers to verify subcellular distribution.
• Cross-reactivity is reported for human, mouse, and rat; before use in other species, check sequence homology and test in pilot experiments.
• ELISA applications may require optimization of coating concentration and blocking conditions; titrate antibody for best signal-to-noise ratio.

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