Anti-ELOVL2 Polyclonal Antibody for WB, ELISA - Q9NXB9

Core Product Specifications and Parameters

Biological Background: ELOVL2 Function and Localization

• ELOVL2 (Very long chain fatty acid elongase 2) is a member of the ELOVL family of fatty acid elongases, also known as elongation of very long chain fatty acids protein 2. It is encoded by the ELOVL2 gene (aliases SSC2, ELG3) in humans.
• This protein functions as a condensing enzyme that catalyzes the first and rate-limiting step in the long-chain fatty acid elongation cycle, primarily within the endoplasmic reticulum membrane.
• ELOVL2 displays high substrate specificity toward polyunsaturated acyl-CoA, particularly C20:4(n-6) acyl-CoA, enabling the synthesis of polyunsaturated very long chain fatty acids (C20- and C22-PUFA).
• These products serve as precursors for membrane lipids and lipid mediators, implicating ELOVL2 in diverse biological processes including membrane structure and signaling.
• The enzyme is localized exclusively to the endoplasmic reticulum membrane as a multi-pass transmembrane protein, consistent with its role in lipid metabolism.
• Tissue expression is predominantly observed in the liver and testis, suggesting specialized roles in hepatic lipid processing and reproductive biology.
• Key functional annotations include fatty acid biosynthesis, lipid metabolism, and transferase activity; the protein contains several transmembrane helices characteristic of ER-bound elongases.

Experimental Guidance and Technical Tips

• The immunogen was designed against amino acids 247–296 of human ELOVL2, a region that may allow recognition of distinct conformational or isoform variants. For Western blot, anticipate a band near the predicted molecular weight of 35 kDa.
• In WB applications, consider using tissue lysates from liver or testis, where endogenous expression is reportedly high, to confirm band specificity.
• For ELISA, optimize antigen coating concentration and antibody dilution using a recombinant ELOVL2 protein or peptide corresponding to the immunogen sequence.
• When using rat samples for cross-reactivity studies, validate that the target sequence is sufficiently conserved; preliminary sequence alignment of the immunogen region is advisable.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for IVD raw materials, including this ELOVL2 antibody for fatty acid metabolism research. As a B2B sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and laboratories from concept to clinic. Reach out to explore how our supply capabilities can accelerate your project.