Anti-Elongin-A2 (TCEB3B) Polyclonal Antibody for WB, ELISA - Q8IYF1

Core Product Specifications and Parameters

Biological Background: Elongin-A2 (TCEB3B) Function and Localization

• Elongin-A2 is also known as RNA polymerase II transcription factor SIII subunit A2 (TCEB3B/TCEB3L) and is a component of the SIII (elongin) complex.
• It functions as a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites.
• Subunit A2 is transcriptionally active independently, but its activity is not enhanced by binding to the elongin BC complex, unlike other SIII subunits.
• The protein is localized exclusively in the nucleus, consistent with its role in transcriptional regulation.
• Elongin-A2 is specifically expressed in testis, suggesting a specialized function in this tissue.
• Keywords associated with the protein include nucleus, transcription, and transcription regulation, highlighting its role in gene expression control.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 534–753 of human Elongin-A2, covering a C-terminal region; consider its relevance for detecting full-length and potential truncated forms.
• This polyclonal antibody is suitable for Western blotting (WB) and ELISA; for WB, validate with testis lysates as a positive control and anticipate a band near 84 kDa.
• Cross-reactivity with mouse and rat expands its utility; verify performance in your specific model system, as expression may be limited to testis.
• For ELISA, optimized coating and detection concentrations should be determined empirically using the recombinant immunogen as a standard.

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