Anti-ELN (Elastin) Rabbit Polyclonal Antibody for IHC-P, ELISA - P15502

Core Product Specifications and Parameters

Biological Background: Elastin Function and Localization

• Elastin (also known as tropoelastin) is a major structural protein, and alternative splicing generates multiple tissue-specific isoforms.
• It provides elasticity to tissues such as the aorta, nuchal ligament, and large arteries, enabling rapid expansion and complete recoil; it also stabilizes arterial structure by regulating proliferation and organization of vascular smooth muscle.
• The protein is secreted into the extracellular space and is a core component of the extracellular matrix.
• Expression is prominent in large arteries, lung, skin, and uterus (outer myometrial smooth muscle and arteriolar tree), as detected at the protein level.
• Post-translational modifications include hydroxylation and disulfide bond formation; the protein is associated with Williams-Beuren syndrome and other matrix-related processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–97) of human elastin; consider validating recognition of full-length and processed forms in relevant sample systems.
• For IHC-P, optimize antigen retrieval methods and include positive control tissues such as aorta or lung to confirm staining specificity.
• For ELISA, use purified recombinant elastin as a standard and titrate antibody concentrations to achieve desirable signal-to-noise ratios.
• Because elastin is a secreted extracellular matrix protein, sample preparation (e.g., tissue homogenization, cell conditioning) may influence detection; verify extraction protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a one-stop sourcing solution for IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-elastin antibody supports research in cardiovascular biology and connective tissue disorders, and our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. We provide reliable procurement support to streamline your assay and workflow development.