Anti-ELMO3 Rabbit Polyclonal Antibody for WB, ELISA - Q96BJ8

Core Product Specifications and Parameters

Biological Background: ELMO3 Function and Localization

• ELMO3 is a member of the engulfment and cell motility (ELMO) protein family, involved in cytoskeletal rearrangements.
• It acts in a complex with DOCK1 and CRK to promote phagocytosis of apoptotic cells and regulates cell migration.
• ELMO3 may enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 toward Rac Rho small GTPases, though this has been shown by similarity.
• The protein is localized to the cytoplasm.
• Alternative splicing of ELMO3 produces multiple isoforms; the protein is also identified as an SH3-binding protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 504-773) of human ELMO3. Validate antibody specificity in relevant human, mouse, or rat cell lysates.
• For Western blotting, a band at ~81 kDa is expected. Optimize blocking and antibody dilution according to your specific protocol.
• This polyclonal antibody may detect multiple isoforms due to alternative splicing; consider using isotype controls and validating with siRNA knockdown or knockout samples.
• For ELISA applications, coating and detection parameters should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and labs with a complete sourcing bridge for IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. As a target implicated in phagocytosis and cell motility, ELMO3-related products support research into apoptosis and cytoskeletal dynamics. Leverage our supply network to secure rare-target raw materials and accelerate your assay development from concept to clinic.