Anti-ELMO2 Rabbit Polyclonal Antibody for WB, ELISA - Q96JJ3

Core Product Specifications and Parameters

Biological Background: ELMO2 Function and Localization

• Engulfment and cell motility protein 2 (ELMO2), also known as protein ced-12 homolog A (hCed-12A), is encoded by the ELMO2 gene in humans.
• It regulates the activity of DOCK guanine nucleotide exchange factors (GEFs), acting in complex with DOCK1/2/4 and CRK to control cytoskeletal rearrangements. Related references: PMID:11595183, PMID:11703939, PMID:20679435, PMID:32651375
• It is critically involved in the phagocytosis of apoptotic cells and cell motility. Related references: PMID:11595183
• Subcellular localization: ELMO2 is present in the cytoplasm, cytosol, and membrane.
• Tissue expression: Widely expressed, with higher expression in skeletal muscle, kidney, and placenta.
• The protein undergoes alternative splicing and phosphorylation, and contains an SH3-binding domain, highlighting its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 42–120) targets the N-terminal region of human ELMO2; for in-house assay development, consider performing peptide competition or domain-specific binding assays.
• For Western blot (WB), validate the antibody in cell lines or tissue lysates known to express ELMO2 (e.g., skeletal muscle, kidney, placenta).
• For ELISA, optimize coating conditions and use appropriate blocking reagents to minimize background; cross-reactivity may require species-specific titration.
• Given the antibody's cross-reactivity with human, mouse, and rat, it is suitable for multi-species studies, but signal intensity should be independently verified for each species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for sourcing IVD raw materials. This anti-ELMO2 antibody supports research into engulfment, cell motility, and phagocytosis, and CamelBio can additionally supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary reagents, and assist in rare target raw-material procurement to accelerate your development pipeline.