Anti-ELMO1 Rabbit Polyclonal Antibody for WB, ELISA - Q92556

Core Product Specifications and Parameters

Biological Background: ELMO1 Function and Localization

• ELMO1 (Engulfment and cell motility protein 1), also known as ced-12 homolog, is a key regulator of DOCK family guanine nucleotide exchange factors (GEFs), including DOCK1, DOCK2, and DOCK5. Related references: PMID:11595183, PMID:12134158, PMID:32651375
• In complex with DOCK and CRK, ELMO1 promotes RAC1 activation, a critical step in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Related references: PMID:11595183, PMID:38857861
• ELMO1 is widely expressed across tissues, with elevated expression in the spleen and placenta, consistent with its role in immune cell function.
• The protein localizes to the cytoplasm and cell membrane, where it participates in signal transduction and cytoskeletal reorganization.
• ELMO1 is subject to post-translational modifications including phosphorylation and acetylation, and contains SH3-binding motifs that mediate protein-protein interactions.
• Alternative splicing of ELMO1 generates multiple isoforms with varying tissue distribution and functional properties.

Experimental Guidance and Technical Tips

• For Western blotting, use a starting sample load of 20–40 µg of total protein lysate from human or mouse cells/tissues, as ELMO1 is an 84 kDa protein with variable expression levels.
• Validate the antibody in your specific sample system and application by including appropriate positive and negative controls; consider using siRNA knockdown or knockout cell lines to confirm band specificity.
• For ELISA applications, this polyclonal antibody may serve as a capture or detection reagent; optimize coating and detection concentrations based on assay format.
• The immunogen corresponds to the C-terminal region (aa 527–727), which may recognize multiple ELMO1 isoforms; note that potential cross-reactivity with ELMO family members (ELMO2, ELMO3) should be evaluated if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-ELMO1 polyclonal antibody is ideal for detecting ELMO1 in phagocytosis, cell motility, and oncogenic signaling studies. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and specialized rare-target raw-material sourcing to streamline your assay development and production.