Anti-ELMO1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q92556

Core Product Specifications and Parameters

Biological Background: ELMO1 Function and Localization

• ELMO1 (Engulfment and cell motility protein 1) is a key regulator of DOCK guanine nucleotide exchange factors (GEFs), promoting RAC1 activation through interactions with DOCK5 and RHOG, and driving cytoskeletal remodeling. Related references: PMID:11595183 PMID:32651375 PMID:38857861
• As a scaffold protein, ELMO1 forms a complex with CRK and DOCK1/2/5 to facilitate cell migration and phagocytosis of apoptotic cells, linking extracellular signals to actin dynamics. Related references: PMID:11595183 PMID:12134158
• The protein is predominantly cytoplasmic but also associates with the cell membrane, enabling its role in membrane-proximal signal transduction during cell movement and engulfment.
• ELMO1 is widely expressed across tissues, with relatively higher levels in the spleen and placenta, suggesting specialized functions in immune surveillance and development.
• Post-translational modifications, including phosphorylation and acetylation, regulate ELMO1 activity, while alternative splicing generates multiple isoforms with potentially distinct functions.
• Annotated keywords such as SH3-binding, phosphoprotein, and proteomics identification further underscore its involvement in signaling networks and proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide spanning residues 628–727 in the C-terminal region of human ELMO1; consider peptide competition or blocking experiments to confirm detection specificity.
• For Western blotting, an ~84 kDa band is expected, but alternative splicing may produce lower-molecular-weight isoforms; validate band identity using relevant cell or tissue lysates.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; known positive controls include spleen or placenta tissue sections.
• For ELISA, test the antibody as capture or detection reagent in appropriate buffer systems and confirm pairing with relevant ELMO1 standards or samples.
• Although the antibody cross-reacts with mouse and rat, sequence homology variations in the immunogen region across species should be verified prior to assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents for ELMO1-related research and diagnostic development. Our capabilities extend to rare target sourcing and customized support, ensuring you have reliable access to the critical raw materials needed for cytoskeletal, apoptosis, and cell motility studies. With a focus on streamlined procurement, we help diagnostic manufacturers and labs accelerate from concept to clinic.