Anti-ELL2 Rabbit Polyclonal Antibody for WB and ELISA - O00472

Core Product Specifications and Parameters

Biological Background: ELL2 Function and Localization

• RNA polymerase II elongation factor ELL2, also known as MRCCAT1, is a transcription regulatory protein.
• Component of the super elongation complex (SEC) and little elongation complex (LEC), which increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing. Related references: PMID:22195968
• Plays a critical role in immunoglobulin secretion in plasma cells by directing alternative mRNA processing, influencing both proximal poly(A) site choice and exon skipping, as well as immunoglobulin heavy chain (IgH) alternative processing.
• Subcellular localization is predominantly nuclear, consistent with its role in transcription regulation.
• Classified as a phosphoprotein and subjected to ubiquitin-like conjugation (Ubl conjugation), suggesting regulatory post-translational modifications.
• Alternative splicing results in multiple isoforms, potentially contributing to functional diversity.
• Possesses a molecular weight of approximately 72 kDa (UniProt: 72324 Da).
• Involved in the regulation of small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-292 of human ELL2, a region that may provide specificity. Validate in your experimental system.
• This antibody is validated for Western blot (WB) and ELISA applications. Optimize antibody dilution and detection conditions for best results.
• Cross-reactivity with rat is reported; consider validating in rat tissue or cell lysates if applicable.
• As a polyclonal antibody, lot-to-lot consistency may vary; confirm performance in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with IVD manufacturers and research laboratories to supply a comprehensive range of raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-ELL2 antibody exemplifies our capability to support transcription elongation research and diagnostic assay development. Leverage CamelBio’s one-stop platform for rare target sourcing and seamless transition from concept to clinical application.