Anti-ELK1 Rabbit Monoclonal Antibody for WB, IP, ELISA - P19419

Core Product Specifications and Parameters

Biological Background: ELK1/Elk-1 Function and Localization

• ELK1 encodes a transcription factor that binds purine-rich DNA sequences and forms a ternary complex with SRF on the serum response element (SRE) of immediate-early genes such as FOS and IER2, mediating transcriptional activation. Related references: PMID:10799319 PMID:7889942 PMID:1630903
• ELK1 belongs to the ETS domain transcription factor family, defined by a conserved winged helix-turn-helix DNA-binding motif.
• Its activity is tightly regulated by JNK and MAPK signaling pathways, linking extracellular stimuli to rapid changes in gene expression.
• Subcellular localization is predominantly nuclear, consistent with its role as a transcription regulator.
• Tissue specificity is observed in lung and testis, though functional relevance may extend to other MAPK-responsive tissues.
• Post-translational modifications include phosphorylation (key for MAPK-mediated activation), glycosylation, and ubiquitin-like conjugation, influencing stability and activity.
• Multiple isoforms are produced by alternative splicing, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 329–428) of human ELK1. Consider using phosphatase inhibitors in lysis buffers to preserve phosphorylation states if analyzing signaling-dependent modifications.
• For western blotting, the predicted molecular weight is approximately 45 kDa; however, phosphorylation and other modifications may cause band shifts or multiple bands.
• Cross-reactivity with mouse and rat ELK1 suggests epitope conservation; validate in the relevant species and tissue type to confirm specificity.
• In ELISA and immunoprecipitation applications, optimize antibody concentrations and buffer conditions to achieve robust signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ELK1 antibody supports transcriptional regulation research and assay development for MAPK pathway targets. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your procurement for reliable diagnostics and translational studies.