Anti-ELK1 Polyclonal Antibody for WB, ELISA - P19419

Core Product Specifications and Parameters

Biological Background: ELK1 Function and Localization

• ETS domain-containing protein Elk-1 (ELK1) is a transcription factor that binds to purine-rich DNA sequences. Related references: PMID:10799319, PMID:7889942

• It forms a ternary complex with serum response factor (SRF) on the serum response element (SRE) of immediate early gene promoters, such as FOS and IER2. Related references: PMID:1630903

• ELK1 activation is regulated by JNK and MAPK signaling pathways, leading to target gene transcription upon stimulation. Related references: PMID:7889942

• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.

• ELK1 expression is enriched in lung and testis tissues.

• Post-translational modifications include phosphorylation, glycosylation, and ubiquitin-like conjugation, which may modulate its activity.

• Alternative splicing of ELK1 generates multiple protein isoforms, potentially diversifying its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 201–297 of human ELK1, a C-terminal region that may be conserved across species and isoforms; consider validating reactivity in your specific experimental system.
• For Western blotting, a band around 45 kDa is expected; validate using appropriate controls and cell lines with known ELK1 expression, such as those from lung or testis.
• For ELISA, optimize coating and detection conditions empirically, as the recombinant immunogen may affect assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated polyclonal antibodies like this anti-ELK1 antibody alongside complementary reagents. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target sourcing, we streamline procurement from concept to clinic. Explore our portfolio for transcription factors and MAPK signaling markers to accelerate your diagnostic development.