Anti-ELF5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UKW6

Core Product Specifications and Parameters

Biological Background: ELF5 Function and Localization

• ELF5 (E74-like factor 5), also known as ESE-2, is an ETS family transcription factor encoded by the ELF5 gene.
• It acts as a transcriptional activator and may regulate later stages of keratinocyte terminal differentiation.
• Isoform 2 binds to DNA sequences with the core motif GGA[AT], and activates the SPRR2A and PSP gene promoters.
• ELF5 is localized to the nucleus, consistent with its role as a transcription factor.
• Expression is restricted to epithelial-rich tissues, including salivary gland, mammary gland, kidney, and prostate; expression is upregulated during keratinocyte differentiation.
• Multiple isoforms exist due to alternative splicing, with isoform 1 and isoform 2 showing differential tissue expression; in kidney only isoform 1 is expressed, while prostate expresses both.
• Several epithelial carcinoma cell lines show lack of ELF5 expression, suggesting potential tumor-suppressive or regulatory roles.
• Keywords: Activator, Alternative splicing, DNA-binding, Nucleus, Transcription regulation.

Experimental Guidance and Technical Tips

• Given the immunogen spans amino acids 40-150, the polyclonal antibody may recognize multiple ELF5 isoforms if the epitope is conserved. For Western blotting, a band around 31 kDa is expected; validate using human, mouse, or rat epithelial cell lysates.
• For immunohistochemistry (IHC-P), normal salivary gland or mammary gland tissues serve as positive controls; expect nuclear staining. Optimize antigen retrieval and antibody dilution empirically.
• For immunofluorescence (IF/ICC), confirm nuclear localization and consider co-staining with epithelial markers. The antibody’s cross-reactivity with mouse and rat enables use in rodent models, but dilutions must be validated per experimental system.
• In ELISA, use recombinant ELF5 protein or cell lysates as capture antigens; proper positive and negative controls should be included.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with comprehensive raw-material sourcing for transcription factor targets like ELF5. Beyond this polyclonal antibody, we offer matched monoclonal antibodies, validated antibody pairs, and essential ancillary reagents for epithelial differentiation studies. Whatever your IVD development needs, from polyclonal to rare target sourcing, our one-stop platform streamlines your path from concept to clinic.