Anti-ELF4 Polyclonal Antibody for IF/ICC and ELISA - Q99607

Core Product Specifications and Parameters

Biological Background: ELF4 Function and Localization

• ELF4 (E74-like factor 4), also referred to as myeloid Elf-1-like factor (MEF), is an ETS-related transcription factor encoded by the ELF4 gene (Gene ID: 2000).
• It functions as a transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3' motif, regulating genes involved in hematopoiesis and immune responses.
• ELF4 transactivates promoters of hematopoietic growth factors, including CSF2 (GM-CSF), IL3, and IL8, and acts synergistically with RUNX1 to activate IL3 expression.
• It plays a critical role in the development and function of natural killer (NK) cells, NK T-cells, and CD8+ T-cells, controlling CD8+ T-cell proliferation and homing via KLF4 and KLF2.
• ELF4 orchestrates inflammatory responses by sustaining the transcription of anti-inflammatory genes (e.g., IL1RN) and negatively regulating pro-inflammatory cytokines such as IL17A, IL1B, IL6, TNFA, and CXCL1. Related references: PMID:34326534 PMID:35266071
• It down-regulates the expression of TREM1, a cell surface receptor that amplifies inflammatory signaling. Related references: PMID:34326534 PMID:35266071
• In addition to its immune functions, ELF4 controls cell senescence in a p53-dependent manner and can promote cellular transformation through inhibition of the p16 pathway.
• Subcellularly, ELF4 localizes to the nucleus and PML nuclear bodies.
• Abundantly expressed in placenta and myeloid leukemia cell lines, with moderate expression in heart, lung, spleen, thymus, peripheral blood lymphocytes, ovary, and colon; lower levels in T-cell lines and absent in brain.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning the N-terminal region (aa 1-200) of human ELF4, which is expected to generate antibodies recognizing endogenous ELF4.
• For immunofluorescence/immunocytochemistry (IF/ICC), nuclear staining is anticipated; optimize permeabilization and fixation protocols for the cell type of interest.
• In ELISA applications, it is recommended to determine the optimal coating antibody concentration and detection system using purified recombinant ELF4 as a standard.
• As a polyclonal antibody, minor batch-to-batch variations may occur; validate performance under your specific experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop platform for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research laboratories from concept to clinic. The ELF4 rabbit polyclonal antibody complements our portfolio of transcription factor and immunology-related reagents, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need consistent supply for immunoassay development or specialized raw materials for inflammation and immune cell research, CamelBio streamlines procurement to accelerate your projects.