Anti-ELAVL4 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P26378

Core Product Specifications and Parameters

Biological Background: ELAVL4 Function and Localization

• ELAVL4 (ELAV-like protein 4), also known as HuD or paraneoplastic encephalomyelitis antigen HuD, is encoded by the ELAVL4 gene and belongs to the ELAV/Hu family of RNA-binding proteins.
• It plays a central role in post-transcriptional regulation by binding to AU-rich elements (AREs) in the 3' UTR of target mRNAs, stabilizing them and protecting from decay; targets include GAP43, VEGF, FOS, CDKN1A, and ACHE. Related references: PMID:10710437, PMID:12034726, PMID:12468554
• Additionally, ELAVL4 regulates alternative splicing, as shown for CALCA pre-mRNA, and modulates translation in a poly(A)- and cap-dependent manner. Related references: PMID:17035636, PMID:10710437
• It is essential for neuronal development, promoting differentiation of neural progenitor cells, neurite outgrowth, dendrite maturation, and synapse function, and contributes to learning and memory. Related references: PMID:12034726, PMID:12468554, PMID:17234598
• Subcellularly, ELAVL4 localizes to the cytoplasm, perikaryon, dendrites, axons, and growth cones, aligning with its roles in mRNA transport and local protein synthesis.
• Expression is predominantly detected in the brain and pancreatic beta cells at the protein level.
• Post-translational modifications include methylation and phosphorylation; it is recognized as the HuD autoantigen in paraneoplastic encephalomyelitis.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal domain (aa 1–100), so epitopes may be accessible in both native and denaturing conditions; consider antigen retrieval for IHC-P and fixation optimization for IF-P.
• This polyclonal antibody can detect endogenous ELAVL4 in human, mouse, and rat samples; validate specificity with knockout controls or peptide blocking where possible.
• For WB, a band near 42 kDa is expected; multiple isoforms or post-translational modifications may produce additional bands.
• Given its role in RNA biology, RNA-binding assays (e.g., RIP) may benefit from UV crosslinking prior to immunoprecipitation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement channel for diagnostic manufacturers and research labs seeking reliable IVD raw materials. This anti-ELAVL4 rabbit polyclonal antibody supports investigations into neurodevelopment, synaptic plasticity, and autoimmune paraneoplastic syndromes. Leverage our supply network for validated antibody pairs, bulk reagents, and rare-target sourcing to accelerate your workflow from discovery to clinical application.