Anti-ELAVL3 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q14576

Core Product Specifications and Parameters

Biological Background: ELAVL3 Function and Localization

• ELAVL3, also known as Hu-antigen C (HuC) or paraneoplastic encephalitis antigen, is a neuron-specific RNA-binding protein.
• It binds to AU-rich elements (AREs) in the 3′-UTR of target mRNAs, such as VEGF mRNA, regulating their stability and translation. Related references: PMID:10710437
• Through its three RNA recognition motifs (RRMs), it may also interact with poly-A tracts, contributing to mRNA regulation.
• ELAVL3 is implicated in neuronal differentiation, maintenance, and synaptic plasticity; it stabilizes GAP43 mRNA and supports spatial learning (By similarity).
• It is expressed specifically in the brain, consistent with its role in neurogenesis and neuronal function.
• The protein is subject to alternative splicing and is annotated with keywords including developmental protein, differentiation, and neurogenesis.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 161–260) lies in a region with potential isoform specificity; confirm reactivity using knockout or knockdown controls.
• For Western blot, expect a band at ~40 kDa; use brain tissue lysates (human, mouse, or rat) as positive controls due to brain‑specific expression.
• For IHC‑P and IF‑P, optimize antigen retrieval and permeabilization conditions; brain sections are recommended starting samples.
• ELISA may be employed for quantitative detection; validate with recombinant protein or tissue extracts.
• Cross‑reactivity with mouse and rat supports use in rodent models; validate the antibody in the relevant species and tissue context.

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