Anti-Elastin Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P15502

Core Product Specifications and Parameters

Biological Background: Elastin Function and Localization

• Elastin (tropoelastin) is a major structural protein of the extracellular matrix, providing elasticity to tissues such as arteries, lung, skin, and ligaments.
• It is a secreted protein that undergoes extensive cross-linking to form elastin fibers, critical for the reversible recoil of these tissues.
• Elastin plays a key role in late arterial morphogenesis by stabilizing vascular structure and regulating smooth muscle cell proliferation and organization (By similarity).
• The protein is expressed in large arteries, lung, skin, and within the myometrial smooth muscle and arteriolar tree of the uterus.
• The ELN gene undergoes alternative splicing, producing multiple isoforms that may contribute to tissue-specific elasticity.
• Post-translational modifications include hydroxylation of proline residues and formation of disulfide bonds, which influence fiber assembly and stability.
• Mutations in ELN are associated with Williams-Beuren syndrome, a developmental disorder affecting the cardiovascular system and connective tissue.
• Keywords: Alternative splicing, Disulfide bond, Extracellular matrix, Hydroxylation, Reference proteome, Repeat, Secreted, Signal, Williams-Beuren syndrome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27-200 of human ELN (NP_001265868.1 isoform). Confirm that your target isoform includes this region when designing experiments.
• For Western blot, note that the predicted molecular weight of the full-length precursor is approximately 68 kDa; mature elastin cross-linked in tissue may exhibit varying migration.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), consider antigen retrieval methods to expose epitopes that may be masked by extensive cross-linking of elastin fibers in tissues.
• Validate the antibody in relevant cell lines or tissues known to express ELN (e.g., aorta, lung, skin) using appropriate positive and negative controls.
• The antibody is reported to cross-react with mouse and rat ELN, but verify cross-reactivity in your specific sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical support, from concept to clinical application. Partner with us to streamline your supply chain for validated antibody pairs, recombinant proteins, and ancillary reagents tailored to your assay development needs. Whether you are developing an ELISA kit for elastin-related disorders or require bulk quantities of rabbit anti-elastin polyclonal antibody, CamelBio offers flexible sourcing solutions to accelerate your project.