Anti-Elastin (ELN) Rabbit Monoclonal Antibody for WB, IHC-P, IF-P, ELISA - P15502

Core Product Specifications and Parameters

Biological Background: Elastin Function and Localization

• Elastin (ELN), also known as tropoelastin, is the major structural protein of elastic tissues such as aorta, lung, and skin, enabling rapid expansion and complete recoil.
• It serves as a molecular determinant of late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle cells.
• The protein is secreted into the extracellular matrix and is a key component of elastic fibers.
• ELN is expressed in large arteries, lung, skin, and within the outer myometrial smooth muscle and arteriolar tree of the uterus.
• The gene undergoes alternative splicing, producing multiple isoforms, and contains repeated hydrophobic and cross-linking domains.
• Post-translational modifications include hydroxylation and disulfide bond formation, critical for structural integrity.
• Mutations in ELN are associated with Williams-Beuren syndrome, a multisystem developmental disorder.
• As an extracellular matrix protein, elastin is also used as a reference proteome in proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 27-200) of human elastin, encompassing the mature protein's start; this region is conserved and suitable for detecting both full-length and processed forms.
• For Western blot (WB), the predicted molecular weight is 68 kDa, but elastin may migrate differently due to extensive cross-linking; consider using reducing and non-reducing conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), ensure proper antigen retrieval because elastic fibers are highly cross-linked.
• Cross-reactivity with rat is reported; validate in the relevant sample system.
• As a monoclonal antibody, it provides high specificity; however, due to alternative splicing, confirm isoform detection if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies like this anti-elastin clone, and bulk ancillary reagents. We support your elastin-related assay development from concept to clinic with reliable raw-material sourcing and technical services.