Anti-Elastin (ELN) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P15502

Core Product Specifications and Parameters

Biological Background: Elastin Function and Localization

• Elastin (ELN) is a major structural protein of elastic tissues, encoded by the ELN gene, and is synthesized as a soluble precursor tropoelastin before cross-linking.
• It serves as the molecular determinant of late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle cells.
• Elastin is essential for the resilience of tissues such as the aorta, nuchal ligament, lung, and skin, allowing them to expand rapidly and recover completely.
• The protein is secreted into the extracellular matrix where it assembles into elastic fibers, contributing to tissue elasticity and structural integrity.
• Tissue-specific expression is observed in the outer myometrial smooth muscle, arteriolar tree of the uterus, large arteries, lung, and skin.
• Post-translational modifications include hydroxylation and formation of disulfide bonds, and the protein features a signal peptide, alternating repeat domains, and undergoes alternative splicing.
• Mutations in ELN are associated with Williams-Beuren syndrome, a multisystem disorder characterized by cardiovascular and connective tissue abnormalities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 27–200 of human ELN, which represents an N-terminal region upstream of the major cross-linking domains; this design may favor recognition of both precursor and mature forms.
• For Western blotting, consider using reducing and non-reducing conditions to characterize monomeric tropoelastin (~68 kDa) and cross-linked elastin polymers; extract extracellular matrix proteins with appropriate chaotropic agents.
• In immunohistochemistry (IHC-P), antigen retrieval may be required for formalin-fixed paraffin sections; validate in tissue sections known to be rich in elastic fibers (e.g., aorta, skin).
• For ELISA, the antibody may be used as a capture or detection reagent; consider pairing with a detection antibody targeting a distinct epitope to avoid steric hindrance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support diagnostic manufacturers and research laboratories. For elastin-related studies in connective tissue disorders and cardiovascular disease, we provide seamless access to this antibody and can source rare-target raw materials to accelerate your development pipeline.