Anti-EIF5A2 Polyclonal Antibody for WB, ELISA - Q9GZV4

Core Product Specifications and Parameters

Biological Background: EIF5A2 Function and Localization

• Encodes eukaryotic translation initiation factor 5A-2 (eIF5A2), a highly conserved protein that facilitates translation elongation and termination, particularly on ribosomes stalled at polyproline motifs and other challenging sequences. Related references: PMID:14622290
• Acts as a ribosome quality control (RQC) cofactor, assisting in peptidyl transfer during CAT tailing on stalled ribosomes, and also participates in mRNA decay and stress-responsive cell wall integrity pathways (By similarity).
• The eIF5A family includes two isoforms in humans: EIF5A1 (constitutively expressed) and EIF5A2 (often overexpressed in cancers), both requiring post-translational hypusination for activity.
• Subcellular location spans the cytoplasm, nucleus, and endoplasmic reticulum membrane, consistent with its roles in translation, nuclear export of mRNAs, and co-translational processes.
• Key post-translational modifications include hypusine formation (essential for activity) and acetylation, with additional RNA-binding capacity; the mature protein is about 17 kDa.
• Tissue expression analysis shows high levels in testis, while overexpression has been documented in ovarian, colorectal, and other cancer cell lines, correlating with poor prognosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length protein (aa 1-153), covering the N-terminal domain critical for ribosome binding and hypusination; this design supports detection of both precursor and mature forms in denaturing gels.
• For western blotting, consider running total cell or tissue lysates under reducing conditions; EIF5A2 migrates near 17 kDa, and loading controls around 15–20 kDa are recommended.
• Validate reactivity in your sample system: this antibody is predicted to cross-react with human, mouse, and rat EIF5A2, but endogenous expression may vary; confirm with positive and negative controls.
• ELISA applications may benefit from using purified recombinant EIF5A2 as a standard and titrating antibody concentrations to optimize signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we streamline the procurement of high-quality IVD raw materials, connecting diagnostic manufacturers and researchers with validated reagents like this anti-EIF5A2 antibody for translation and oncology studies. Our integrated supply network offers optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—helping you move from concept to clinic with confidence. Inquire about custom formulations, matched pairs, or full-suite support for your next diagnostic development project.